The Museum consists of three spaces: an introductory hall and two exhibition areas.
In the first room an educational summary on the physical and cultural evolution of Man is presented.
The second room includes two distinct sectors.
The first, the largest, is reserved for materials coming from the most important prehistoric Italian sites, from the Archaic Palaeolithic until the Bronze Age. A specific itinerary is dedicated to prehistoric art.
The second sector is dedicated to African prehistory, of which the Museum holds the most extensive collection in Italy.
A space dedicated to the prehistory of the Florentine area is set up alongside.